BTC-3200D Cell and Tissue Culture Techniques

This 140-hour introduces the theory and practices required to successfully initiate and maintain animal cell culture. Topics include aseptic techniques, growth environment, routine maintenance of cell cultures, cryopreservation, transfection, and cell counting. Other topics include cloning techniques, suspension culture, serum-free culture, evaluation of cryopreserved cells, extraction of DNA from mammalian cells, specialized culture techniques, and various applications. Upon completion, students should be able to demonstrate the knowledge and skills required to grow, maintain, and manipulate cells in culture. Prerequisites: 1) Must be 18 years of age or older AND 2) BioPharma Lab Skills Certificate OR of Bioscience Associate's degree or higher (unofficial OK). Students must submit proof of prerequisites prior to registration. Please email biotechce@durhamtech.edu for questions regarding programs or registration process.